Genome Annotation

1. Structural Annotation - identifying functional elements in the genome.
2. Functional Annotation - attaching biological information to these elements.

• Open reading frames (ORFs) predicted during genome assembly require experimental confirmation.
• The Sequence Ontology provides a structured controlled vocabulary for sequence annotation.

• Functional annotation of gene products = Gene Ontology (GO) annotation.
• Initially, predicted ORFs have no functional literature and GO annotation relies on computation methods (rapid).
• Functional literature exists for many genes/proteins prior to genome sequencing.
• GO annotation does not rely on a completed genome sequence!